scholarly journals Asian-American variants of human papillomavirus type 16 have extensive mutations in theE2 gene and are highly amplified in cervical carcinomas

1999 ◽  
Vol 83 (4) ◽  
pp. 449-455 ◽  
Author(s):  
Leonora Casas ◽  
Silvia C. Galvan ◽  
Rosa M. Ordo�ez ◽  
Nora Lopez ◽  
Miriam Guido ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Asian American ◽  
Cervical Carcinomas ◽  
Human Papillomavirus Type 16

scholarly journals Complete Genome Sequences of Eight Human Papillomavirus Type 16 Asian American and European Variant Isolates from Cervical Biopsies and Lesions in Indian Women: TABLE 1 

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Paramita Mandal ◽  
Bornali Bhattacharjee ◽  
Shrinka Sen ◽  
Amrapali Bhattacharya ◽  
Rahul Roy Chowdhury ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Asian American ◽  
Complete Genome ◽  
Etiological Agent ◽  
Genome Sequences ◽  
Indian Women ◽  
Human Papillomavirus Type 16 ◽  
European Variant

Human papillomavirus type 16 (HPV16), a member of thePapillomaviridaefamily, is the primary etiological agent of cervical cancer. Here, we report the complete genome sequences of four HPV16 Asian American variants and four European variants, isolated from cervical biopsies and scrapings in India.

scholarly journals Overlapping YY1- and aberrant SP1-binding sites proximal to the early promoter of human papillomavirus type 16

1999 ◽  
Vol 80 (8) ◽  
pp. 2097-2101 ◽  
Author(s):  
Xiao-Ping Dong ◽  
Herbert Pfister
Keyword(s):  
Human Papillomavirus ◽  
Binding Site ◽  
Binding Sites ◽  
Cellular Proteins ◽  
Long Control Region ◽  
Hpv 16 ◽  
Cervical Carcinomas ◽  
Human Papillomavirus Type 16 ◽  
E6 And E7 ◽  
Transcription Factor Yy1

Transcription of oncogenes E6 and E7 of human papillomavirus type 16 (HPV-16) from the P97 promoter is regulated by viral and cellular proteins. The transcription factor YY1 represses transcription through binding to cognate sequences in the long control region (LCR). In HPV-16 DNA from cervical carcinomas, mutations of YY1-binding sites have been identified that increase P97 activity 3–6-fold. A second, SP1-binding site has now been identified in the HPV-16 LCR (nt 7842–7847), which overlaps the YY1-binding site at positions 7840–7848. A point mutation within this YY1 site in viral DNA from a cervical cancer, previously shown to prevent YY1 binding, was shown to increase SP1 binding and P97 activity 4·7-fold. An engineered mutant eliminating SP1 binding showed only 1- to 1·6-fold increased P97 activity. It is concluded that competition between SP1 and YY1 for DNA binding plays a major role in YY1 repression mediated by the binding site at positions 7840–7848.

scholarly journals Influence of human papillomavirus type 16 (HPV-16) E2 polymorphism on quantification of HPV-16 episomal and integrated DNA in cervicovaginal lavages from women with cervical intraepithelial neoplasia

2008 ◽  
Vol 89 (7) ◽  
pp. 1716-1728 ◽  
Author(s):  
Naoufel Azizi ◽  
Jessica Brazete ◽  
Catherine Hankins ◽  
Deborah Money ◽  
Julie Fontaine ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Viral Load ◽  
Cervical Intraepithelial Neoplasia ◽  
Asian American ◽  
Intraepithelial Neoplasia ◽  
Hpv 16 ◽  
Viral Loads ◽  
Human Papillomavirus Type 16 ◽  
Immunodeficiency Virus ◽  
Intraepithelial Lesion

Integrated human papillomavirus type 16 (HPV-16) viral loads are currently estimated by quantification with real-time PCR of HPV-16 E6 (RT-E6 and HPV-16 PG) and E2 (RT-E2-1) DNA. We assessed the influence of HPV-16 E2 polymorphism on quantification of integrated HPV-16 DNA in anogenital specimens. HPV-16 E2 was sequenced from 135 isolates (123 from European and 12 from non-European lineages). An assay targeting conserved HPV-16 E2 sequences (RT-E2-2) was optimized and applied with RT-E6 and RT-E2-1 on 139 HPV-16-positive cervicovaginal lavages collected from 74 women [58 human immunodeficiency virus (HIV)-seropositive and 16 HIV-seronegative]. Ratios of HPV-16 copies measured with RT-E2-2 and RT-E2-1 obtained with African 2 (median=3.23, range=1.92–3.49) or Asian–American (median=3.78, range=1.47–37) isolates were greater than those obtained with European isolates (median=1.02, range=0.64–1.80; P<0.02 for each comparison). The distribution of HPV-16 E2 copies measured in 139 samples with RT-E2-2 (median=6150) and RT-E2-1 (median=8960) were different (P<0.0001). The risk of high-grade cervical intraepithelial neoplasia (CIN-2,3) compared with women without CIN was increased with higher HPV-16 total [odds ratio (OR)=2.17, 95 % confidence interval (CI)=1.11–4.23], episomal (OR=2.14, 95 % CI=1.09–4.19), but not for HPV-16 integrated viral load (OR=1.71, 95 % CI=0.90–3.26), after controlling for age, race, CD4 count, HIV and HPV-16 polymorphism. The proportion of samples with an E6/E2 ratio >2 in women without squamous intraepithelial lesion (7 of 35) was similar to that of women with CIN-2,3 (5 of 11, P=0.24) or CIN-1 (5 of 14, P=0.50). HPV-16 E2 polymorphism was a significant factor that influenced measures of HPV-16 integrated viral load.

scholarly journals Tumourigenesis Driven by the Human Papillomavirus Type 16 Asian-American E6 Variant in a Three-Dimensional Keratinocyte Model

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e101540 ◽  
Author(s):  
Robert Jackson ◽  
Melissa Togtema ◽  
Paul F. Lambert ◽  
Ingeborg Zehbe
Keyword(s):  
Human Papillomavirus ◽  
Asian American ◽  
Three Dimensional ◽  
Human Papillomavirus Type 16

Use of Up-Converting Phosphor Reporters in Lateral-Flow Assays to Detect Specific Nucleic Acid Sequences: A Rapid, Sensitive DNA Test to Identify Human Papillomavirus Type 16 Infection

2001 ◽  
Vol 47 (10) ◽  
pp. 1885-1893 ◽  
Author(s):  
Paul Corstjens ◽  
Michel Zuiderwijk ◽  
Antoinette Brink ◽  
Shang Li ◽  
Hans Feindt ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Nucleic Acid ◽  
Viral Infections ◽  
Lateral Flow ◽  
Blind Test ◽  
Dna Test ◽  
Cervical Carcinomas ◽  
Human Papillomavirus Type 16 ◽  
Lateral Flow Assays ◽  
Nucleic Acid Sequences

Abstract Background: A lateral-flow (LF) device using the new reporter up-converting phosphor technology (UPTTM) was applied to DNA (hybridization) assays for the detection of specific nucleic acid sequences, thereby aiming to perform the test outside well-equipped laboratories. The methodology reported here is sensitive and provides a rapid alternative for more elaborate gel electrophoresis and Southern blotting. In a preliminary study, it was applied to screen for the presence of human papillomavirus type 16 (HPV16) in a defined series of cervical carcinomas. Methods: A LF assay was used to capture haptenized DNA molecules and hybrids, which were immunolabeled (before LF) with 400-nm UPT particles. These particles emit visible light after excitation with infrared in a process called up-conversion. Because up-conversion occurs in only the phosphor lattice, autofluorescence of other assay components is virtually nonexistent. Results: The use of the UPT reporter in LF-DNA tests, as compared with colloidal gold, improved the detection limit at least 100-fold. UPT LF-DNA tests were successfully applied to detect (in a blind test) the presence of HPV16 in DNA extracts obtained from cervical carcinomas. Test results matched 100% with previous characterization of these carcinomas. Conclusions: The use of UPT in LF assays to detect specific nucleic acids provides low attamole-range sensitivity. Hybridization and consecutive detection of PCR-amplified HPV16 sequences were successful in a background of 10 μg of fish-sperm DNA. The sensitivity of UPT detection in these complex mixtures indicates that detection of viral infections without PCR or other amplification technique is achievable.

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